Correction: Quantitative Assessment of Antibody Internalization with Novel Monoclonal Antibodies against Alexa Fluorophores

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There are a number of errors in the headings for Fig 1B, “Quenching by anti-Alexa Fluor mAbs.” “Beads coated with 1C1-A488” and “Beads stained with 1C1-A488” should be “Beads coated with 1C1-A594” and “Beads stained with 1C1-A594”. Please see the corrected Fig 1 here. There are errors in the equation in the second to last sentence of the penultimate paragraph of the subsection “Flow cytometry-based internalization assay using cell surface fluorescence quenching.” The correct sentence should be: Internalized fluorescence was calculated from quenched and non-quenched sample data by correcting for incomplete surface quenching: 1 – (N1 –Q1)/(N1 – (N1Q0/N0)) with N1 = Unquenched MFI at each time point (t1); Q1 = Quenched MFI at t1; Q0 = Quenched MFI for the sample kept on ice (t0); N0 = Unquenched MFI at t0. The publisher apologizes for the error. There are errors in the equation in the first sentence of the last paragraph of the subsection “Flow cytometry-based internalization assay using cell surface fluorescence quenching.” The correct sentence should be: Internalized fluorescence was plotted and a curve fit was obtained in Prism by nonlinear regression with the one-phase association equation: Y = Y0 + (Plateau – Y0) (1 – exp(-K x)) with Y0 = 0 when X (time) is zero and Plateau = 100. The publisher apologizes for the error.

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Quantitative Assessment of Antibody Internalization with Novel Monoclonal Antibodies against Alexa Fluorophores

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Correction: Quantitative Assessment of Antibody Internalization with Novel Monoclonal Antibodies against Alexa Fluorophores

There is an error in the Correction published on May 15, 2015. The corrected headings for Fig 1B are “Cells stained with 1C1-A488” and “Cells stained with 1C1-A594”, not “Beads stained with 1C1-A488” and “Beads stained with 1C1-A594”. The publisher apologizes for the error. The correct text is: There are a number of errors in the headings for Fig 1B, “Quenching by anti-Alexa Fluor mAbs.” “Beads...

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عنوان ژورنال:

دوره 10  شماره 

صفحات  -

تاریخ انتشار 2015